Journal: Experimental Biology and Medicine

Article Title: Curcumin-enhanced elvitegravir therapy mitigates neuroinflammation and cognitive deficits in EcoHIV mice

doi: 10.3389/ebm.2025.10758

Figure Lengend Snippet: Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

Article Snippet: Primary antibody incubation was carried out overnight at 4 °C using the following antibodies: NeuN rabbit polyclonal antibody (1:1000, Proteintech, Cat# 26975-1-AP), synaptophysin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 67864-1-Ig), GFAP rabbit polyclonal antibody (1:1000), L1CAM rabbit polyclonal antibody (Proteintech, Cat# 20659-1-AP), and β-actin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 66009-1-Ig) as an internal loading control.

Techniques: Infection, Western Blot, Expressing

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Reagents and instruments used in the study

Article Snippet: Mouse monoclonal anti-synaptophysin antibody , Proteintech Group (Rosemont, IL, USA) , 60191-1-1g (RRID: AB_10915965).

Techniques: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Treatment with NaHS inhibits the dysregulation of neuroplasticity induced by Quin. (A, B) Immunofluorescence images showing colocalization of PSD95 (red, CoraLite594 fluorescent dye) and NeuN (green, CoraLite488 fluorescent dye) in nine coronal sections from three mice per group. PSD95 expression was significantly upregulated in neuronal cells in the Quin + NaHS group compared with the Quin group, and this effect was reversed by the addition of ML385. Scale bar: 20 μm. (C) Western blot was used to measure PSD95, SYT1, and Syn expression levels 6 days after Quin striatal injection. Quantification of PSD95, SYT1, and Syn expression levels ( n = 3 per group). The values are reported as mean ± SD. *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). NeuN: Neuronal nuclei; PSD95: postsynaptic density protein 95; Quin: quinolinic acid; Syn: synaptophysin; SYT1: synaptotagmin 1.

Article Snippet: Mouse monoclonal anti-synaptophysin antibody , Proteintech Group (Rosemont, IL, USA) , 60191-1-1g (RRID: AB_10915965).

Techniques: Immunofluorescence, Expressing, Western Blot, Injection

Journal: Investigative Ophthalmology & Visual Science

Article Title: Multiterminal En Plaque Motor Endplates in Extraocular Muscles Are Conserved Across Vertebrate Species

doi: 10.1167/iovs.66.4.77

Figure Lengend Snippet: Light microscopy images and schematic illustrations of different types of motor endplates in rabbit EOMs. Single en plaque motor endplates ( A – C , arrowheads ), multiple en grappe motor endplates ( D – F , arrowheads ), multiterminal en plaque motor endplates ( G – J , arrows ), and sporadic complex en grappe motor endplates ( K – N , arrows ) were labeled with α-BTx ( red in B , E , H , and L ) and antibodies against neurofilament and synaptophysin (NF + SYN; green in A , D , G , and K ). The contour of myofibers was outlined by immunolabeling with the antibody against laminin ( white in I and M ). Merged images in C , F , J , and N . Scale bars : 50 µm.

Article Snippet: A mixture of mouse monoclonal antibodies SY38 against synaptophysin (Boehringer Mannheim, Indianapolis, IN, USA) and M0762 against neurofilament 70 kDa (Dako Denmark A/S, Glostrup, Denmark) were used to detect NMJs by labeling the pre-synaptic region in EOMs of rabbits and mice.

Techniques: Light Microscopy, Labeling, Immunolabeling

Journal: Investigative Ophthalmology & Visual Science

Article Title: Multiterminal En Plaque Motor Endplates in Extraocular Muscles Are Conserved Across Vertebrate Species

doi: 10.1167/iovs.66.4.77

Figure Lengend Snippet: Confocal images and schematic illustrations of three types of motor endplates in mouse EOMs. Single en plaque motor endplates ( A – D , arrowheads ), multiple en grappe motor endplates ( E – G , arrows ), and multiterminal en plaque motor endplates ( H – J , arrows ) were labeled with α-BTx ( red in A , E , and H ) and antibodies against neurofilament and synaptophysin (NF + SYN; white in B and I ), and myofibers were labeled with antibody against obscurin ( green in C and G ). Merged images showed in D , G , and J . Scale bars : 50 µm in D and G , 25 µm in J .

Article Snippet: A mixture of mouse monoclonal antibodies SY38 against synaptophysin (Boehringer Mannheim, Indianapolis, IN, USA) and M0762 against neurofilament 70 kDa (Dako Denmark A/S, Glostrup, Denmark) were used to detect NMJs by labeling the pre-synaptic region in EOMs of rabbits and mice.

Techniques: Labeling